The CENP-B homolog, Abp1, interacts with the initiation protein Cdc23 (MCM10) and is required for efficient DNA replication in fission yeast

Table 2 Synthetic Interactions between Δabp1 and DNA replication mutants.

*S. pombe* strains	Gene product	25°C	30°C	31°C	32°C	33°C	34°C	36°C
Wild type		++	++	++	++	++	++	++
Δabp1		+	+	+	+	+	+	+
*cdc23-M36*	S phase factor	++	++	++	-	-	-	-
Δabp1 cdc23-M36		+	-	-	-	-	-	-
Δabp1Δcbh2		+	+	+	+	+	+	+
Δabp1Δcbh2 cdc23-M36		+	-	-	-	-	-	-
Δcbh1Δcbh2		+	+	+	+	+	+	+
Δcbh1Δcbh2 cdc23-M36		+	+	+/-	-	-	-	-
*cdc20-P7*	DNA polymerase	++	++	++	++	++	-	-
Δabp1 cdc20-P7	ε catalytic subunit	+	+	+	+	+	-	-
*cdc20-M10*	DNA polymerase	++	++	++	++	++	+/-	-
Δabp1 cdc20-M10	ε catalytic subunit	+	+	+	+	+	+	-
*cut5-T401*	S phase initiator	++	++	++	++	++	-	-
Δabp1 cut5-T401		+	+	+	+	+	-	-
*cdc30-2H4*	ORC complex	++	++	++	+/-	-	-	-
Δabp1 cdc30-2H4		+	+	+/-	-	-	-	-
*cdc18-K46*	S phase initiator	++	++	++	++	++	+	-
Δabp1 cdc18-K46		+	+	+	+	+/-	-	-
*cdc21-M68*	MCM2-7 complex	++	++	++	+	+	-	-
Δabp1 cdc21-M68	subunit 4	+	+	+	+/-	-	-	-
*sna41-912*	S phase initiator	++	++	+/-	-	-	-	-
Δabp1 sna41-912		+	+	+/-	-	-	-	-
*orp2-2*	ORC complex	++	++	++	++	+	-	-
Δabp1 orp2-2		+	+	+	+	+	+	-
*orp2-7*	ORC complex	++	++	++	++	++	++	+
Δabp1 orp2-7		+	+	+	+	+	+	-
*cdc1-P13*	DNA polymerase	++	++	-	-	-	-	-
Δabp1 cdc1-P13	δ small subunit	+	+	+	-	-	-	-
*cdc6-23*	DNA polymerase	++	-	-	-	-	-	-
Δabp1 cdc6-23	δ catalytic subunit	+	+/-	-	-	-	-	-
*cdc27-P11*	DNA polymerase	++	++	++	++	-	-	-
Δabp1 cdc27-P11	δ associated factor	+	+	+	+	+/-	-	-
*cdc17-M75*	DNA ligase	++	++	+	+/-	-	-	-
Δabp1 cdc17-M75		+	+	+	+	+	+/-	-

Note: ++ = normal growth; + = slow growth; +/- = very slow growth, elongated; = dead, elongated

ISSN: 1747-1028