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Table 2 Synthetic Interactions between Δabp1 and DNA replication mutants.

From: The CENP-B homolog, Abp1, interacts with the initiation protein Cdc23 (MCM10) and is required for efficient DNA replication in fission yeast

S. pombe strains Gene product 25°C 30°C 31°C 32°C 33°C 34°C 36°C
Wild type   ++ ++ ++ ++ ++ ++ ++
Δabp1   + + + + + + +
cdc23-M36 S phase factor ++ ++ ++ - - - -
Δabp1 cdc23-M36   + - - - - - -
Δabp1Δcbh2   + + + + + + +
Δabp1Δcbh2 cdc23-M36   + - - - - - -
Δcbh1Δcbh2   + + + + + + +
Δcbh1Δcbh2 cdc23-M36   + + +/- - - - -
cdc20-P7 DNA polymerase ++ ++ ++ ++ ++ - -
Δabp1 cdc20-P7 ε catalytic subunit + + + + + - -
cdc20-M10 DNA polymerase ++ ++ ++ ++ ++ +/- -
Δabp1 cdc20-M10 ε catalytic subunit + + + + + + -
cut5-T401 S phase initiator ++ ++ ++ ++ ++ - -
Δabp1 cut5-T401   + + + + + - -
cdc30-2H4 ORC complex ++ ++ ++ +/- - - -
Δabp1 cdc30-2H4   + + +/- - - - -
cdc18-K46 S phase initiator ++ ++ ++ ++ ++ + -
Δabp1 cdc18-K46   + + + + +/- - -
cdc21-M68 MCM2-7 complex ++ ++ ++ + + - -
Δabp1 cdc21-M68 subunit 4 + + + +/- - - -
sna41-912 S phase initiator ++ ++ +/- - - - -
Δabp1 sna41-912   + + +/- - - - -
orp2-2 ORC complex ++ ++ ++ ++ + - -
Δabp1 orp2-2   + + + + + + -
orp2-7 ORC complex ++ ++ ++ ++ ++ ++ +
Δabp1 orp2-7   + + + + + + -
cdc1-P13 DNA polymerase ++ ++ - - - - -
Δabp1 cdc1-P13 δ small subunit + + + - - - -
cdc6-23 DNA polymerase ++ - - - - - -
Δabp1 cdc6-23 δ catalytic subunit + +/- - - - - -
cdc27-P11 DNA polymerase ++ ++ ++ ++ - - -
Δabp1 cdc27-P11 δ associated factor + + + + +/- - -
cdc17-M75 DNA ligase ++ ++ + +/- - - -
Δabp1 cdc17-M75   + + + + + +/- -
  1. Note: ++ = normal growth; + = slow growth; +/- = very slow growth, elongated; = dead, elongated