Figure 3

Representative trace of position of single Eg5 dimer moving in vitro. Record shows motion of a bead-attached Eg5 dimer held in an optical trap, and walking along microtubules in 8.1-nm steps. Position (light blue) and smoothed position (dark blue) are plotted as a function of time; dotted lines are placed every 8.1 nm to guide the eye. Experimental conditions: 2 mM ATP, 4 pN load applied toward the microtubule plus-end (assisting motion).