Figure 4
From: Modification of Cul1 regulates its association with proteasomal subunits
Ubiquitylated Cul1 binds the S5a subunit of the 19S proteasome. (A) Pulldown of 35S Cul1 using GST alone (lane 1), GST-S5a (lane 2), GST-αlpha 6 (lane 3) or GST-αlpha 7 (lane 4). (B) Pulldown of 35S wild-type Cul1 (lanes 1 and 2) or 35S Cul1(K720A) (lanes 3 and 4) using GST alone (lanes 1 and 3) or GST-S5a (lanes 2 and 4). Ten percent input of 35S wild-type Cul1 and 35S Cul1(K720A) are shown in lanes 5 and 6, respectively. (C) Pulldown of 35S wild-type Cul1 in vitro translated in the absence (lanes 1 and 2) or presence (lanes 3 and 4) of methylated ubiquitin (MeUb) using GST alone (lanes 1 and 3) or GST-S5a (lanes 2 and 4). Ten percent input of 35S Cul1 in vitro translated in the absence or presence of MeUb are shown in lanes 5 and 6, respectively.