Figure 5

CD59 and CD55 expression in G0-G1 and G2-M subpopulations. Data is depicted as representative means for three independent experiments. Unsynchronized Bcap37 cells (Figure 5a) and MCF7 cells (Figure 5b) were grown to confluency and maintained in media supplemented with 2 mM L-glutamine (Unsynch). Synchronized cells were grown to confluency in normal media and then maintained in media without glutamine for 48 hours, followed by 8 hours in media supplemented with 2 mM L-glutamine (Gln Restored). Cells were then harvested, treated with propidium iodide, incubated with either FITC-conjugated antibodies to CD59 or to CD55 and prepared for flow cytometric sorting and analysis.