Figure 2
Human 14-3-3γ stimulates abnormal proliferation in eye imaginal discs. Third instar larvae were either heat shocked as indicated or left untreated and the eye imaginal discs dissected and processed to incorporate BrdU as described in the methods section. A mouse anti-BrdU monoclonal antibody was used to detect BrdU-labeled cells. An anti-Elav antibody was used to stain differentiating proneural photoreceptor cells in the region posterior to the morphogenetic furrow. Confocal images are depicted. BrdU incorporation marks replicating cells (BrdU), Elav expression signifies differentiated proneuronal cells (Elav). Merged images show the relative placement of replicating cells relative to differentiating cells (Merged). In photomicrograph "a" the letter A marks the anterior region of the eye disc. The letter P marks the posterior region of the disc. In photomicrograph "g" a white arrow points to replicating cells in the posterior compartment of the eye imaginal disc. The letter P marks the posterior compartment of the disc. In photomicrograph i "MF" marks the morphogenetic furrow (the white bar). In "J" and "K" panels, BrdU-incorporated "S" phase cells were shown (white arrows) in the 2nd mitotic wave region ("J") behind of morphogenetic furrow and in the differentiating proneuronal cell region ("K"), respectively. In photomicrograph"l", a combined image of anti-BrdU and anti-elav stained cells is shown. The white scale bars, on the right top corners in the photomicrographs "J" and "K", show 20 μm.