Figure 4

Human 14-3-3γ induces increased cell proliferation in the morphogenetic furrow region of eye imaginal discs. Third instar larvae were collected and processed for incorporation of BrdU as described previously and the imaginal discs dissected and examined using confocal microscopy. GFP expression (GFP) is shown in green and is due to the presence of UAS-nlsGFP. BrdU incorporation (BrdU) is shown in red. Merged images are shown on the right. (A) Shows control animals (w; GMR-Gal4/UAS-nlsGFP). (B) Shows imaginal disc from transgenic line (yw; GMR-Gal4, UAS-h1433γ/UAS-nlsGFP). In photomicrograph "b" the letter P marks the posterior side of the imaginal disc and the letter A marks the anterior side. In photomicrographs "b" and "e" white rectangular boxes mark the position of the second mitotic wave. MF (the white bars, "f" and "h") marks morphogenetic furrow. The letter S (the white arrow) in photomicrograph "g", refers to cells in "S" phase. The letter P marks posterior compartment of the eye disc ("h"). The size of white scale bars on the top right corners in photomicrographs "g" and "h" is 20 μm.