Abnormal vulva and male tail development in the san-1(ok1580) animal. L1 larvae were grown at 20°C for three days and 1-day old adults were analyzed using DIC microscopy. Wild-type hermaphrodites (A), san-1(ok1580) hermaphrodites (B-D), wild-type males (E) and san-1(ok1580) males (F-H) were analyzed for morphologic defects. White arrows point to vulva defects including tissue damage (B), abnormal morphology (C), and protruding vulva (D). Black arrows point to defects observed in the male tail, including tissue damage (F), ray and fan defects (G), and abnormal tissue structure (H). Bar = 25 μm.