Figure 8

The hcp-1(RNAi) 2-cell embryo does not have a significant difference in mitotic timing in comparison to wild-type 2-cell embryos. Live cell imaging with a spinning disc confocal microscope was used to analyze mitotic progression in hcp-1(RNAi);tbg-1::GFP;pie-1::GFP::H2B and control animals. The AB and P cells of a 2-cell embryo were analyzed. In the P cell of a wild-type and hcp-1(RNAi) 2-cell embryo the time it took (seconds) to transition from nuclear envelope break down (NEB) to formation of the metaphase plate, formation of the metaphase plate to anaphase onset and NEB to anaphase onset was determined. The time between NEB of the AB cell and NEB of the P cell was also determined. Each experiment was conducted at 21°C (n = 7 embryos). The P value was determined using a standard student t test.