Figure 2
From: Morphogenesis signaling components influence cell cycle regulation by cyclin dependent kinase
cdc28-E217V cells exhibit specific genetic interactions with cell-cycle regulators. A) Growth of cdc28 mutant yeast cells containing genomic substitution of E217V transformed with high-copy plasmids harboring CKS1, CAK1, CLN1, CLB2 or no insert, or a centromeric plasmid harboring CDC28. B) p13Suc1 beads-pull downs using yeast protein extract prepared from wild-type or cdc28-E217V at 30°C. Blots were probed with anti-PSTAIRE CDK antibody. C) Inviability and elongated morphology of clb2 cdc28-E217V double mutant segregants.