Figure 6
From: Morphogenesis signaling components influence cell cycle regulation by cyclin dependent kinase
Increased Swe1 protein levels mediate cdc28-E217V defects. A) p13Suc1 beads pull-downs of Tyr19-phosphorylated and unphosphorylated Cdc28 from protein extracts prepared from swe1 or mih1 deletion strains. B) Western blot analysis of Myc-tagged Swe1 protein levels from wild-type and cdc28-E217V cells grown to log phase at 25°C and shifted to 37°C, or kept at 25°C for 4 h.