Morphogenesis signaling components influence cell cycle regulation by cyclin dependent kinase

Cell Division

Table 1 Genetic comparison of temperature sensitive alleles containing mutations in the Cks1 binding domain

Cks1 Binding Domain Mutations	Suppression by *CKS1* overexpression	Suppression by *flo8*
V113A, P231L	+	-
K151E, F245L, S248G	+	-
F245V, D244E, R10I, I218T	++	-
C121R, N232I	-	-
S213R, S216R, I224T	-	-
L228S, L275H	+	-
Y8C, F212L	+	+
N155I, N238G	+	+
E70G, S197T, N232K	++	+
E12G, T54A, I235V	-	+
Q32R, K151E, S248G	-	-
E217V	++	+
D215V, R253C	+	+
R43G, W236R	+	-
N238D	++	+

HIS3-marked plasmids (pRS413) containing temperature sensitive cdc28 alleles were introduced into a CDC28 shuffle strain. Transformants were subjected to 5-FOA treatment to evict the wild type copy of CDC28 and assayed for growth at 25°C and 35°C. If growth of a given allele was significantly improved by deletion of FLO8 or by overexpression of CKS1, then suppression is indicated by "+". A sign of "-" indicates no change.

ISSN: 1747-1028