Figure 1
From: RBX1/ROC1-SCF E3 ubiquitin ligase is required for mouse embryogenesis and cancer cell survival
Substrate ubiquitination by RBX1-SCF E3 ubiquitin ligase: Cullin-1 at its N-terminus binds to Skp1 and an F-box protein, which recognizes protein substrates, and at its C-terminus binds to RBX1. RBX1, on the other hand, binds to Cullin-1 using its N-terminus and an E2 ubiquitin conjugating enzyme using its C-terminal RING domain. Together, RBX1-cullin-1 catalyzes the ubiquitin transfer from E2 to protein substrates.