Figure 4

Representative immunoblots of benign (A) and malignant (B) tissues. The proteins from benign and malignant tissues were extracted and separated by SDS-PAGE. The proteins were then transferred to a nitrocellular membrane and placed in a manifold which separate the membrane into 20 channels. Each channel was blotted with 2-4 primary antibodies. The secondary antibody was detected by chemiluminescence. The positive signal as well as correct location ensures the correct identification of the proteins.