Figure 5
From: Impact of steroid hormone signals on Drosophila cell cycle during development
EcR signaling is required for dmyc expression in wing imaginal discs. (A-D) Discs with control clones to show dmyc-lacZ (w67c 23P{lacW}l(1)G0354G 0354; [109]) enhancer trap activity throughout the cycling cells of the wing pouch, with reduced β-gal antibody staining within the G1 arrested cells of the ZNC (A) β-gal staining (red) (B) GFP (green) positively marks clones (C) Overlay of GFP and β-gal, (D) Merge of GFP, β-gal with DNA (blue) to show the nuclei of apical cells in the wing pouch. (E-H) UAS-EcRAdN clones in the dmyc-lacZ background. (E) β-gal staining (red) (F) GFP (green) marked UAS-EcRAdN clones (G) Overlay of GFP and β-gal. (H) Merged image of GFP, β-gal and DNA (blue).