Table 1 Mutant alleles of S. pombe chk1

From: Regulation of Chk1

Allele	Mutation	Reference
chk1 Δ	Null	[6, 8]
chk1-T15	ND¹	[6]
chk1-1	E92D	[46]
chk1–2	I484T	[46]
chk1-NC18	ND	[9]
chk1-NC73	ND	[9]
chk1-NE3	ND	[9]
-	K38A	[44]
-	G108S	[44]
-	F118L, S311F	[44]
-	N142D	[44]
-	L144P	[44]
-	D155G	[44]
-	D155A	[44]
-	D155E²	[44]
-	D363G, D469G	[44]
-	S345A	[23, 24]
-	S345D	[24]
chk1-ts1	E472D^3,7	[27]
chk1-ts2	E111R⁴	[27]
chk1-ts3	L151S⁴	[27]
chk1-ts4	I389S⁴	[27]
chk1-ts5	ΔI484⁴	[27]
-	L291A⁵	[42]
-	L295A⁵	[42]
-	L299A⁵	[42]
-	L303A⁵	[42]
chk1–4A	K377A, R378A, K381A, K382A⁶	[42]
-	D363G	[43]
-	D469G	[43]
-	Δ280–496	[45]
-	Δ327–496	[45]
-	Δ393–496	[45]
-	Δ444–496	[45]
-	Δ467–496	[45]
-	Δ486–496	[45]
-	Δ359–361^3,7	[45]
-	R394A⁷	[45]
-	R394A, R397A	[45]
-	F398A, Y399A	[45]
-	Δ394–400	[45]
-	E472A	[45]
-	ΔE472	[45]
-	ΔF477	[45]
-	Δ473–477	[45]
-	G468A, D469A, P470A, E472A	[45]

Notes: ¹Not determined; ²dominant-negative when overexpressed; ³temperature sensitive loss-of-function; ⁴temperature sensitive loss-of-function when expressed from a multi-copy plasmid, but non-functional when expressed from the chk1 locus; ⁵disrupts 14-3-3 binding; ⁶blocks nuclear localization; ⁷gain-of-function when expressed from the attenuated nmt1 promoter.

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