Human eIF3f expression is cell growth related in A549 cells. A) Western blot (WB) analysis of eIF3f expression in quiescent A549 cells (G0) and proliferating asynchronous cultures (A). Tubulin WB is shown to document equal protein loading. B) Promoter activity of the upstream sequences of eIF3f by promoter driven luciferase activity in quiescent A549 cells (G0) and proliferating asynchronous cultures (A). Luciferase activity with the empty vector pGL3 was below 10 RLU/μg of protein. Data expressed as mean ± SE; * p < 0.01 with respect to G0, n ≥ 3.