Figure 2From: Senescent mouse cells fail to overtly regulate the HIRA histone chaperone and do not form robust Senescence Associated Heterochromatin Foci Senescent MEFs stain more intensely with histone variant macroH2A. (a) Human IMR90 fibroblasts transduced with either control or H-RasG12V were stained for histone variant macroH2A and DNA (DAPI). Inactive X chromosome is enriched in macroH2A in female cells (arrow). (b) Control or H-RasG12V-transduced senescent MEFs were stained for histone variant macroH2A. Results were quantitated using metamorph and expressed as histograms and box plots: The red line is the median (50th percentile); the box itself encompasses the 25th and 75th percentiles (Inter Quartile Range (IQR)); the whiskers are the most extreme data points within 1.5 × IQR; crosses outside the whiskers are outliers. (c) Images of cells stained as in (b). White arrows indicate a cell with increased macroH2A stain.Back to article page