Figure 1
From: The WW-HECT protein Smurf2 interacts with the Docking Protein NEDD9/HEF1 for Aurora A activation
Smurf2 physically interacts with NEDD9 during mitosis. Extracts were prepared from HeLa cells that were either asynchronous (A) or synchronized at mitosis by the thymidine-nocodazole protocol (S), and 400 μg proteins were immunoprecipitated (IP) with the indicated antibodies or normal immunoglobulin (IgG), followed by immunoblotting (IB) for the indicated proteins. Forty micrograms of whole cell extracts (10% WCE) were also subjected to IB for comparison.