Figure 3
From: The WW-HECT protein Smurf2 interacts with the Docking Protein NEDD9/HEF1 for Aurora A activation
Depletion of Smurf2 results in enhanced polyubiquitination and proteasomal degradation. (A) Decline in NEDD9 induced by siSmurf2 (Sm2) was rescued by treatment with proteasomal inhibitors. siRNA-transfected HeLa cells were treated with either 2 μM MG132, 5 μM lactacystin (LACT) or equivalent volume DMSO for 4 h. (B) siSmurf2 leads to polyubiquitination of NEDD9. NEDD9 was immunoprecipitated from HeLa cells transfected with siRNA and treated with MG132.