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Figure 3 | Cell Division

Figure 3

From: Regulated degradation of the APC coactivator Cdc20

Figure 3

Overexpressed CDH1-m11 lowers Cdc20 levels, but does not clear it. Either HA-CDH1 or HA-CDH1-m11 was induced using deoxycorticosterone in cycling strains with a GAL4-Mineralocorticoid receptor fusion (containing the DNA binding domain of the former, and ligand binding domain of the latter, rendering GAL responsive genes inducible by exogenous mineralocorticoids [38]). Levels of endogenously expressed 18MYC-Cdc20 and HA-Cdh1 were followed by immunoblot, with a nonspecific band reactive with the anti-HA antibody serving as a loading control.

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