Figure 3From: Regulated degradation of the APC coactivator Cdc20 Overexpressed CDH1-m11 lowers Cdc20 levels, but does not clear it. Either HA-CDH1 or HA-CDH1-m11 was induced using deoxycorticosterone in cycling strains with a GAL4-Mineralocorticoid receptor fusion (containing the DNA binding domain of the former, and ligand binding domain of the latter, rendering GAL responsive genes inducible by exogenous mineralocorticoids [38]). Levels of endogenously expressed 18MYC-Cdc20 and HA-Cdh1 were followed by immunoblot, with a nonspecific band reactive with the anti-HA antibody serving as a loading control.Back to article page