Figure 5
From: GSK3 regulates the expressions of human and mouse c-Myb via different mechanisms
Depletion of endogenous GSK3β affects the transcriptional expression of human c-Myb and promotes the transrepression of γ-globin in K562 cells. K562 cells were transfected with an siRNA for GSK3β or a control siRNA. At 48 h after transfection, the protein levels of GSK3β and c-Myb were analyzed by immunoblotting (A, B, C) and the mRNA levels of c-Myb and γ-globin were measured by quantitative RT-PCR (D, E). The immunoblots in (A) show representative data. Each relative protein or mRNA level was calculated as the mean ± s.d. from six independent experiments.