Self-organization of intracellular gradients during mitosis

Cell Division

Table 1 FRET reporters used to study intracellular mitotic gradients

Name	Probe for:	Structure	Reference
YRC	Binding/release of Ran	YFP-*Ran Binding Domain*-CFP	Kalab [4]
YIC	Binding/release of Importin Beta cargo	YFP-*Importin-β Binding Domain*-CFP	Kalab [4]
COPY	Tubulin binding to Stathmin	CFP-*OP18/Stathmin*-YFP	Niethammer [1]
Alexa488-Ran	Ran/Importin Beta interaction	*Ran*-Alexa 488	Caudron [7]
Cy3-Importin-β	Ran/Importin Beta interaction	*Importin-β*-Cy3	Caudron [7]
RANGO	Binding/release of Importin Beta cargo	EYFP- *Importin-β Binding Domain*-Ceru.	Kalab [2]
ABAR	Aurora B activity	*Targeting-CFP- FHA2-Substrate*-YFP	Lampson et. al [3]

ABAR, Aurora B activity reporter; Ceru., cerulean; CFP, cyan fluorescent protein; EYFP, enhanced yellow fluorescent protein; RANGO, Ran-regulated Importin beta cargo; YFP, yellow fluorescent protein; YIC, YFP-Importin-Beta Binding Domain-CFP; YRC, YFP-Ran Binding Domain-CFP.

ISSN: 1747-1028