Mutation to the APC subunit Apc5 reveals antagonistic interactions between Gcn5 and Hda1. (A) Serial 10-fold dilutions of each strain were spotted onto YPD plates from left to right and incubated at the temperatures shown. (B) Serial dilutions using strains expressing the indicated plasmids were spotted onto SD-ura plates containing either 2% glucose or 2% galactose, and grown at 30°C for 2 and 3 days, respectively. (C) Protein lysates were prepared from the mutants shown and characterized by Westerns using the antibodies indicated. Antibodies against GAPDH were used as load controls.