Expression of PDS1 , an APC antagonist, is specifically elevated in apc5CA hda1 Δ cells at 37°C. (A) Northern analyses were conducted on total RNAs extracted from the various mutants and probed with sequences derived from the coding regions of the genes indicated. RDN37-2 (RDN1) was used as a loading control. All bands from the 30°C experiments (B) and the 37°C experiments (C) were quantified by ImageJ and normalized to the RDN1 signal. Densitometry was performed on two Northern experiments and two reverse transcriptase experiments. The data was combined and the means and standard errors were plotted.