Figure 3

Histone H3 acetylation at promoter regions is elevated specifically in apc5^CA hda1 Δ cells at 37°C. (A) ChIP was performed using lysates derived from the mutants shown and antibodies against total H3 or H3 acetylated at both K9 and K14. A mock treatment lacking antibody was used as a control. Once the crosslinks were reversed and DNA recovered, "end point" PCR was performed using primers against the genes shown that amplified 200 bp regions of the promoter. 10% of the reaction was used as input. (B) Two independent experiments were quantified, with the means and standard errors represented graphically, as previously described [66, 70].