Figure 1

The G ₁ phase Cdks and the E2Fs regulate various steps in the centrosome duplication cycle. Various evidence suggests that the G₁ phase Cdks directly phosphorylate NPM, CP110 and Mps1 to regulate centrosome licensing and duplication. The dotted line reflects the fact that even though Plk4 is not a direct target of Cdk2, introduction of a dominant-negative Cdk2 construct renders it ineffective in triggering centriole reduplication. The figure reflects how the E2F activators E2F1, E2F2 and E2F3 influence the centrosome duplication cycle by controlling the transcriptional levels of cyclins E, A, D, and Cdk2. The figure also reflects how E2F3 and E2F4 repress cyclin E and Nek2 to influence the centrosome cycle.