Figure 2

Swe1 is degraded faster in cdc5^T23A cells. Cdc5Δ cells expressing Cdc5^WT or cdc5^T23A were synchronized in S-phase, released and rearrested at metaphase. Rabbit anti-Clb2 was a kind gift from A. Amon. Swe1 was 6myc tagged by integration of pRS305 Swe1-6myc cut with SnaBI [26] and detected using 9E10 mouse anti-myc (a kind gift of M. Goldberg).