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Table 1 Yeast strains used in this study

From: The loop-less tmCdc34 E2 mutant defective in polyubiquitination in vitro and in vivo supports yeast growth in a manner dependent on Ubp14 and Cka2

Strain Genotype Reference
862 MAT a dsk2::Kan R his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 [73]
3195 MAT a ubp14::Kan R his3Δ1 leu2Δ0 met15Δ 0 ura3Δ0 [73]
13195 MATα ubp14::Kan R his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 [73]
BL2 MAT a [22]
EJ758(YIL035c) MAT a his3-Δ200 leu2-3,112 ura3-52 pep4::HIS3 pYEX4T-+rec::CKA1 [74]
EJ758(YOR061w) MAT a his3-Δ200 leu2-3,112 ura3-52 pep4::HIS3 pYEX4T-+rec::CKA2 [74]
FY24 MATα ura3-52 trp1-Δ63 leu2-Δ1 F. Winston
FY78 MAT a his3Δ200 F. Winston
KS418 MAT a CDC34 tm ura3 leu2 trp1 lys2 ade2 ade3 [22]
MT1901 MATα mfa1Δ::pMFA1-HIS3 can1Δ ura3Δ0 leu2Δ0 his3Δ1 lys2Δ0 M. Tyers
RC6 MAT a CDC34 tm (NAT1) ura3 leu2 trp1 ade2 ade3 [22]
RC21 MAT a/α CDC34tm(NAT1)/CDC34 ura3/ura3 leu2/leu2 trp1/TRP1 lys2/LYS2 ade2/ADE2 ade3/ADE3 his3/HIS3 MFA1/mfa1Δ::pMFA1-HIS3 can1/CAN1 [22]
RC29 MATα cdc34tm(NAT1) mfa1Δ::pMFA1-HIS3 his3Δ ura3Δ leu2Δ can1Δ [22]
RC94 MATαCDC34(NAT1) mfa1Δ::pMFA1-HIS3 his3Δ1 leu2Δ0 ura3Δ0 can1Δ [22]
RC85 MAT a CDC34tm(NAT1) [22]
RC171 MAT a/α CDC34tm(NAT1)/CDC34 ubp14::KanR /UBP14 mfa1Δ::pMFA1-HIS3/MFA1 his3Δ/his3Δ ura3/ura3Δ0 leu2/leu2Δ0 can1/CAN1 met15Δ0/MET15 This study
RC172 MAT a/α CDC34(NAT1)/CDC34 ubp14::KanR /UBP14 mfa1Δ::pMFA1-HIS3/MFA1 his3Δ/his3Δ ura3/ura3Δ0 leu2/leu2Δ0 can1/CAN1 met15Δ0/MET15 This study
RC173 MAT a/α CDC34tm(NAT1)/CDC34 cka2::KanR /CKA2 mfa1Δ::pMFA1-HIS3/MFA1 his3Δ/his3Δ ura3/ura3Δ0 leu2/leu2Δ0 can1/CAN1 met15Δ0/MET15 This study
RC174 MAT a/α CDC34(NAT1)/CDC34 cka2::KanR /CKA2 mfa1Δ::pMFA1-HIS3/MFA1 his3Δ/his3Δ ura3/ura3Δ0 leu2/leu2Δ0 can1/CAN1 met15Δ0/MET15 This study
RC175 MAT a/α CDC34tm(NAT1)/CDC34 rps7b::KanR /RPS7B mfa1Δ::pMFA1-HIS3/MFA1 his3Δ/his3Δ ura3/ura3Δ0 leu2/leu2Δ0 can1/CAN1 met15Δ0/MET15 [22]
RC176 MAT aCDC34(NAT1)/CDC34 rps7b::KanR /RPS7B mfa1Δ::pMFA1-HIS3/MFA1 his3Δ/his3Δ ura3/ura3Δ0 leu2/leu2Δ0 can1/CAN1 met15 0/MET15 [22]
YL10-1 MAT a cdc34-2 leu2Δ1 ura3-52 trp1Δ63 his3Δ Gal+ [17]