Table 1 Yeast strains used in this study

862

MAT a dsk2::Kan ^R his3Δ1 leu2Δ0 met15Δ0 ura3Δ0

[73]

3195

MAT a ubp14::Kan ^R his3Δ1 leu2Δ0 met15Δ 0 ura3Δ0

[73]

13195

MATα ubp14::Kan ^R his3Δ1 leu2Δ0 met15Δ0 ura3Δ0

[73]

BL2

MAT a

[22]

EJ758(YIL035c)

MAT a his3-Δ200 leu2-3,112 ura3-52 pep4::HIS3 pYEX4T-+rec::CKA1

[74]

EJ758(YOR061w)

MAT a his3-Δ200 leu2-3,112 ura3-52 pep4::HIS3 pYEX4T-+rec::CKA2

[74]

FY24

MATα ura3-52 trp1-Δ63 leu2-Δ1

F. Winston

FY78

MAT a his3Δ200

F. Winston

KS418

MAT a CDC34 ^tm ura3 leu2 trp1 lys2 ade2 ade3

[22]

MT1901

MATα mfa1Δ::pMFA1-HIS3 can1Δ ura3Δ0 leu2Δ0 his3Δ1 lys2Δ0

M. Tyers

RC6

MAT a CDC34 ^tm (NAT1) ura3 leu2 trp1 ade2 ade3

[22]

RC21

MAT a/α CDC34^tm(NAT1)/CDC34 ura3/ura3 leu2/leu2 trp1/TRP1 lys2/LYS2 ade2/ADE2 ade3/ADE3 his3/HIS3 MFA1/mfa1Δ::pMFA1-HIS3 can1/CAN1

[22]

RC29

MATα cdc34tm(NAT1) mfa1Δ::pMFA1-HIS3 his3Δ ura3Δ leu2Δ can1Δ

[22]

RC94

MATαCDC34(NAT1) mfa1Δ::pMFA1-HIS3 his3Δ1 leu2Δ0 ura3Δ0 can1Δ

[22]

RC85

MAT a CDC34^tm(NAT1)

[22]

RC171

MAT a/α CDC34^tm(NAT1)/CDC34 ubp14::Kan^R /UBP14 mfa1Δ::pMFA1-HIS3/MFA1 his3Δ/his3Δ ura3/ura3Δ0 leu2/leu2Δ0 can1/CAN1 met15Δ0/MET15

This study

RC172

MAT a/α CDC34(NAT1)/CDC34 ubp14::Kan^R /UBP14 mfa1Δ::pMFA1-HIS3/MFA1 his3Δ/his3Δ ura3/ura3Δ0 leu2/leu2Δ0 can1/CAN1 met15Δ0/MET15

This study

RC173

MAT a/α CDC34^tm(NAT1)/CDC34 cka2::Kan^R /CKA2 mfa1Δ::pMFA1-HIS3/MFA1 his3Δ/his3Δ ura3/ura3Δ0 leu2/leu2Δ0 can1/CAN1 met15Δ0/MET15

This study

RC174

MAT a/α CDC34(NAT1)/CDC34 cka2::Kan^R /CKA2 mfa1Δ::pMFA1-HIS3/MFA1 his3Δ/his3Δ ura3/ura3Δ0 leu2/leu2Δ0 can1/CAN1 met15Δ0/MET15

This study

RC175

MAT a/α CDC34^tm(NAT1)/CDC34 rps7b::Kan^R /RPS7B mfa1Δ::pMFA1-HIS3/MFA1 his3Δ/his3Δ ura3/ura3Δ0 leu2/leu2Δ0 can1/CAN1 met15Δ0/MET15

[22]

RC176

MAT a/α CDC34(NAT1)/CDC34 rps7b::Kan^R /RPS7B mfa1Δ::pMFA1-HIS3/MFA1 his3Δ/his3Δ ura3/ura3Δ0 leu2/leu2Δ0 can1/CAN1 met15 0/MET15

[22]

YL10-1

MAT a cdc34-2 leu2Δ1 ura3-52 trp1Δ63 his3Δ Gal+

[17]