Timing of anaphase A chromatid-to-pole movement in Drosophila S2 cells. (A) Stills from a movie following anaphase A chromatid-to-pole movement in an S2 cell stably expressing histone 2B (green) and -α-tubulin (red). Time is expressed as min:sec and scale bar represents 5 μm. (B) Graph illustrating the timing of anaphase A chromatid to pole motility relative to anaphase B spindle elongation. Note that time point 0 in the graph does not correspond to that in the stills.