Figure 1

Hypersensitivity of slt2 mutant strain to genotoxic stress. A) 10-fold serial dilutions from exponentially growing cultures of wild type (W303-1a) and slt2 (JCY1062) strains were spotted onto YPD medium containing 100 mM hydroxyurea, 0.025% MMS or 5 μg/mL phleomycin or were exposed to UV radiation (35 J/m²). Plates were incubated at 25°C for 3 days. B) Aliquots from exponentially growing cultures of wild type (W303-1a), slt2 (JCY1062) and mec1 sml1 (JCY1039) strains were incubated for 90 min. at the indicated doses of HU, MMS and phleomycin or were exposed to different doses of UV radiation. Cells were plated on YPD and the percentage of surviving cells relative to untreated controls was determined. C) Aliquots from exponentially growing cultures of wild type (SEY6211) and slt2 (JCY193) strains were incubated in the presence of 0.4 M HU, 0.02% MMS, 5 μg/mL phleomycin for 1 hour or were exposed to UV radiation (10 J/m²). Cell survival relative to untreated controls was determined.