Figure 6

Analysis of ribonucleotide reductase (RNR) function in the slt2 mutant strain. A) Exponentially growing cultures of the wild type W303-1a strain were split and incubated for 120 min in the absence or presence of 200 mM hydroxyurea or 0.04% MMS. The protein level of the different subunits of the RNR enzyme was analyzed by western blot. A non-specific band labelled with an asterisk that cross-react with the antibody is shown as loading control. B) Exponentially growing cultures of the wild type W303-1a strain were split and incubated for 120 min in the absence or presence of 0,04% MMS. The cellular content of dATP, dCTP and dGTP were determined in crude cell extracts. Similar results were obtained with cells incubated for 60 min in the presence of HU or MMS.