Figure 3

Over-expression of Hos2p results in growth inhibition and in phenotypic defects related to morphogenesis and/or cytokinesis. (A) Strains expressing hos2 from the nmt1/41/81 series of promoters were streaked to EMM media in the presence (repressed) or absence (de-repressed) of thiamine and incubated at 30°C for two days. (B) Colony morphology of strains treated as in A. Bar, 50 μm (C) Representative examples of the pleiotropic cellular morphology exhibited by cells expressing hos2 from the nmt1 promoter; (i) cells with a rounded, de-polarized appearance, ii) cells with misplaced septa, iii) cells with abnormally excessive and mis-localized septal deposition, iv) cells with multiple septa v) highly elongated cells containing multiple aberrant deposits of septal material, vi) elongated and branched cells. Bar, 10 μm.