Figure 4

Hos2p localizes to both the cytoplasm and the nucleus and physically interacts with Set3p, Snt1p, and Hif2p. (A) Cells expressing the Hos2-GFP fusion protein were grown to mid-log phase at 30°C in YES media, fixed, and then stained with DAPI and observed using the DAPI and GFP filter sets. Bar, 5 μm. (B) Cells expressing the indicated fusion proteins were grown to mid-log phase in YES, lysed under native conditions, and subjected to anti-HA immunoprecipitations. Both total lysates and immunoprecipitates were resolved by SDS-PAGE and immunoblotted with antibodies specific for the Myc epitope.