DNA methylation status in double silenced IMR90 cells. On the left is shown the Slot-blot analysis of 5-methylcytosine (5-MeC) antibody. DNA samples of control, siDNMT1/p14 and siDNMT1/p53 IMR90 cells were spotted onto Hybond-N membrane and blotted with 5-MeC antibody. Spotted DNA quantity was revealed by methylene blue staining. On the right is shown the graph illustrating differences in 5-MeC quantity between control, siDNMT1/p14 and siDNMT1/p53 IMR90 cells. Data are expressed related to control cells following DNA normalization with methylene blue.