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Table 1 DNA methylation status after DNMT1 silencing in HCT116

From: Bypass of cell cycle arrest induced by transient DNMT1 post-transcriptional silencing triggers aneuploidy in human cells

Sample % Methylated DNA
HCT116 ctrl 5,9% ± 0,4
HCT-siDNMT1 1,95% ± 1,06
  1. DNA extracted from cells was treated with anti-5-methylcytosine antibody, stained with the "capture antibody" and the "detection antibody" and then quantified by an EliSa-like reaction. DNA extracted from HCT-siDNMT1 cells appeared demethylated in comparison to that extracted from HCT116 cells.
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Cell Division

ISSN: 1747-1028

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