Figure 4

Morphological changes in the nuclei during luteolin- and colchicine-induced apoptosis in Mgc-803 cells detected by AO/EB staining. After treatment with colchicine and luteolin at 5, 10, and 20 μM, Mgc-803 cells were stained with AO/EB (100 μg/μM) and observed under fluorescence microscopy. A: Negative control (without treatment). B, C: Positive control; treatment with VP-16 and HCPT (20 μM) for 24 h, respectively. D–F: Treatment with colchicine (5, 10, and 11 μM, respectively) for 24 h. G–I: Treatment with colchicine (5, 10, and μM, 12 respectively) for 36 h. J–L: Treatment with luteolin (5, 10, and 20 μM, respectively) for 24 h. M–O: Treatment with luteolin (5, 10, and 20 μM, respectively) for 48 h.