Inhibition of the staining of the MAb Tit1 5 H1.1 by specific peptides. (A) Location of MAb Tit1 5 H1.1 target antigen (titin) in Bowes melanoma cell line on the 2nd day of cultivation. The cells were fixed with 4% PFA and permeabilized with Tritone X-100 before staining for titin with MAb Tit1 5 H1.1. Specific staining was visualized with Alexa Fluor 594 conjugated to goat anti-mouse IgG (red), obj. 100x . (B) Negative staining of cells with secondary antibody. (C) Cells of the same culture as in A were stained with MAb Tit1 5 H1.1 but inhibited by specific full-sized peptide (N-AVNKYGIGEPLESDSVVAK-C). Note a marked reduction of staining intensity and no centriole staining,obj. 100x. (D and E) Cells were stained with MAb Tit1 5 H1.1 and inhibited by subpeptides N-AVNKYG-C and N-PLESDSVC, respectively. Note a marked reduction of staining intensity with inhibition using N-AVNKYG-C and no staining reduction with peptide N-PLESDSV-C, obj.100x. Cell nuclei were counterstained with DAPI (blue).