Replication of somatic micronuclei in bovine enucleated oocytes

Cell Division

Table 1 Evaluation of different combinations of spindle inhibitors and mitomycin for the induction of somatic micronuclei

Spindle inhibitor	Mit	% Micronucleate cells / total cells	Media of micronuclei / cell
DMC 0.05 μg/ml	-	10^a	7
DMC 0.05 μg/ml	+	25.5^b	7
DMC 0.1 μg/ml	-	6.25^a	7
DMC 0.1 μg/ml	+	4.25^a	8
Col 0.05 μg/ml	-	3.75^a	6
Col 0.05 μg/ml	+	3.25^a	6

Ovarian bovine fibroblasts were blocked in metaphase by incubation with 0.05 or 0.1 μg/ml DMC (Demecolcine); or 0.05 μg/ml Col (Colchicine) for 46 or 48 h. In order to complete mitotic arrest, some cells were then incubated with 2 mg/ml Mit (Mitomycin) for 2 h.
^a,b: Values with different superscripts in the same column differ significantly (p≤0.05).

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