Identification of new cell size control genes in S. cerevisiae

Cell Division

Table 1 Summary of cell size mutants

	Gene	ORF	Cell size (fL)	Budding %	G1%	S/G2/M%	Human homolog	E-value	Function
*LOGARITHMIC*
	WT		101.7	56	38	62
*whi*	rpl36bΔ	YPL249C-A	66.0**	40	62	38	RPL36	2e-26	Translation
	mrpl49Δ	YJL096W	66.8**	45	59	41	RFT1	0.16	Translation
	cbs1Δ	YDL069C	71.2**	43	45	55	TLR5	0.28	Translation
	rpl42aΔ	YNL162W	73.9**	46	50	50	RPL36AL	2e-51	Translation
		YJR114W	77.1*	54	53	47	-	--	Unknown
	rom2Δ	YLR371W	80.2**	60	55	45	ARHGEF3	3e-16	GEP for Rho
	rpl16bΔ ^#	YNL069C	83.5*	52	44	56	L13a	5e-76	Translation
*uge*	ecm9Δ	YKR004C-A	135.9*	62	37	63	KCNS2	0.060	Cell Wall Organization
	ctr9Δ	YOL145C	128.5*	36	33	67	CTR9	2e-48	Transcription
*SATURATED*
	WT		49.7	3	96	4
*uge*	ctr9Δ	YOL145C	76.8*	11	93	7	CTR9	2e-48	Transcription

A summary of cell size mutants, grouped together under logarithmic and saturated conditions, is shown. Data includes the gene, open reading frame (ORF) of the gene, mean cell size in femtoliters (fL), budding index values, cell cycle distribution in logarithmic phase obtained by FACS, human homolog, E-values and cellular function associated with each gene. Statistical differences in mean cell size values were calculated using unpaired t-test (**p<0.0001, *p<0.05). ^# All genetic strains are homozygous diploids except for rpl16bΔ which is a heterozygous diploid. Thus, rpl16bΔ is a haploinsufficient cell size mutant. Note that YJR114W is a dubious ORF.

ISSN: 1747-1028