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Table 2 Summary of cell cycle effects

From: Identification of new cell size control genes in S. cerevisiae

  Daughter Mother Overall
Strain Birth size B1 size G1 (min) B2 size CT B1 size CT1 B2 size CT2 B3 size G1 (min) CT RGR
WT 5.4 5.8 32 6.0 83 6.3 75 6.4 74 6.4 4 77 100%
mrpl49Δ 4.2 5.1 172 5.2 167 5.5 140 5.6 154 5.7 30 154 51%
rpl42aΔ 4.4 5.1 94 5.4 131 5.5 97 5.5 115 5.6 8 114 58%
yjr114wΔ 4.5 5.4 136 5.5 183 5.8 136 5.9 137 6.0 25 152 67%
rpl36bΔ 4.6 5.4 131 5.6 158 5.7 143 5.8 139 5.8 24 147 67%
rom2Δ 4.6 5.1 89 5.3 135 5.6 115 5.6 111 5.7 4 120 64%
cbs1Δ 4.6 5.6 141 5.9 208 6.0 129 6.1 150 6.2 26 162 69%
rpl16bΔ 5.0 5.5 47 5.8 124 5.9 94 6.0 100 6.1 6 106 83%
ctr9Δ 5.5 6.5 130 6.8 153 7.1 110 7.2 145 7.3 20 136 116%
ecm9Δ 6.1 6.6 41 7.0 127 7.0 114 7.2 107 7.3 8 116 155%
  1. Data gathered from microscopic analyses of >30 individual cells over a 6–10 hour time course is presented. Cell diameters are in microns. In each case, the birth diameter of daughter cells is shown. In addition, the size at which cells bud for the first time (B1) and subsequent buds (B2, etc.) are also provided. The length of G1-phase is directly measured as the time cells remain unbudded. Cycle time (CT) is measured from the time a cell first buds until its subsequent bud. Overall cycle time (CT) is shown as the average of one daughter CT and the first two mother cell cycle times. The relative growth rate (RGR) is a measure of the total cell volume produced per generation and is determined by calculating the change in volume of mother cells in each generation added, to the volume of daughter cells produced. For volume calculations, cells are assumed to resemble spheres and the formula 4/3πr3 is used. Wild type (WT) cells are set to 100% and all mutants are subsequently normalized to this value.