WT + | Mean | N | SE | G1% | S/G2/M% | Budding index |
|---|
Empty Vector
| 78 | 11 | 1 | 56.99 | 40.56 | 44.3 |
ECM9
| 62** | 11 | 1 | 52.21 | 47.79 | 60.3 |
CTR9
| 80 | 14 | 1 | 54 | 46 | 65.7 |
MRPL49
| 68** | 11 | 2 | 52.38 | 47.62 | 51.9 |
YJR114W
| 68* | 13 | 2 | 72.53 | 27.47 | 44.4 |
RPL42A
| 68** | 13 | 1 | 52.24 | 47.76 | 57.7 |
RPL16B
| 72** | 13 | 1 | 44.31 | 55.69 | 62.3 |
CBS1
| 69* | 14 | 2 | 72.64 | 24.46 | 45.7 |
RPL36B
| 73 | 6 | 4 | 59.31 | 40.09 | 41.6 |
- Over-expression analysis in wild type cells. This table illustrates the effects of over-expressing the new size mutant genes in wild type yeast cells. Data includes the average cell size from logarithmic cultures, number of replicates, standard error, % of cells in G1 and S/G2/M phases as determined by flow cytometry, and the budding index values. Statistical differences in mean cell size values were calculated using unpaired t-test (**p<0.0001, *p<0.0051). All analyses were carried out in -Ura Raf/Gal media using GAL-inducible vectors. Vector alone (pB63) was used as a control. Note: We were unable to generate a GAL-ROM2 inducible vector.
