Figure 6

Ectopic activation of Cdh1 causes mitotic slippage. (A, B) Ectopic activation of Cdh1 causes mitotic slippage. Cells of a strain SCU398 (CEN-GFP) harboring plasmids pSCU878 (pGAL-CDH1) or pSCU145 (empty vector) were released from α-factor into nocodazole-containing SGalR medium (time 0), as described in Figure 1. Cell images and data were obtained after the 6 h time point. (C) Ectopic activation of Cdh1 and Cdc14 causes securin degradation. Cells of strain SCU2755 (PDS1-HA3) harboring plasmids pSCU145 (empty vector), pSCU1575 (pGAL-CDH1) or pSCU1576 (pGAL-CDC14) were released from α-factor into nocodazole-containing SGalR medium (time 0), as described in Figure 1. (D) The same cells as described in (C) were arrested using nocodazole and then galactose was added (time 0). (E, F) Ectopic activation of Cdc14 causes securin degradation in a manner dependent on Cdh1. Cells of strains SCU396 (CEN-GFP) and SCU1700 (cdh1Δ CEN-GFP) harboring the plasmid pSCU802 (pGAL-CDC14) were arrested in metaphase by nocodazole treatment for 3 h and then galactose was added for 3 h. Cells of the strain SCU396 (CEN-GFP) with plasmid pSCU134 (empty vector) were used as the control.