Table 1 Yeast strains used in this study
From: APC/C-Cdh1-dependent anaphase and telophase progression during mitotic slippage
Name (Alias) | Description (Source) |
|---|---|
SCU15 (W303a) | Mata ura3 his3 leu2 trp1 ade2 can1 (lab stock) |
SCU151 (bub2Δ) | SCU893 bub2::hphMX4 (this study) |
SCU396 (CEN-GFP) | SCU893 his3::GFP12-LacI12-NLS::HIS3 trp1::LacOx256-TRP1 (this study) |
SCU397 (bub2Δ CEN-GFP) | SCU396 bub2::loxP (this study) |
SCU398 (CEN-GFP) | SCU893 ura3::tetO2x112::URA3 leu2::tetR-GFP-NLS::LEU2 (this study) |
SCU399 (bub2Δ CEN-GFP) | SCU398 bub2::hphMX (this study) |
SCU404 (bub2Δ GAL-SCC1-RRDD CEN-GFP) | SCU397 leu2::GAL1-SCC1-R180D/R268D-HA3::LEU2 (this study) |
SCU408 (bub2Δ PDS1-HA3) | SCU151 PDS1-HA3::URA3 (this study) |
SCU410 (bub2Δ MET3-CDC20 CEN-GFP) | SCU399 MET3-CDC20::TRP1 (this study) |
SCU893 (bar1Δ) | SCU15 bar1::hisG (U. Surana) |
SCU1226 (cdh1Δ CEN-GFP) | SCU15 ura3::tetO::URA3 leu2::tetR::LEU2 cdh1::HIS3 [63] |
SCU1228 (cdh1Δ) | SCU15 cdh1::kanR [63] |
SCU1336 (bub2Δ cdh1Δ CEN-GFP) | SCU1226 bub2::kanMX (this study) |
SCU1337 (MAD2-GFP) | SCU893 mad2::kanM X [pMAD2-GFP] (this study) |
SCU1338 (bub2Δ MAD2-GFP) | SCU151 mad2::kanMX [pMAD2-GFP] (this study) |
SCU1700 (cdh1Δ CEN-GFP) | SCU1228 trp1::LacOx256:TRP1 his3::HIS3p-GFP13-LacI12NLS::HIS3 (this study) |
SCU2755 (PDS1-HA3) | SCU893 pds1::PDS1-HA3::URA3 (this study) |
SCU2834 (bub2Δ cdh1Δ PDS1-HA3) | SCU1336 ura3 pds1::PDS1-HA3::URA3 (this study) |