Figure 4
From: Regulation of cell proliferation and cell density by the inorganic phosphate transporter PiT1
Over-expression of hPiT1 increases proliferation and cell density of MC3T3-E1 cultures. A) Cell counts. MC3T3-E1-LPiT1SN (LPiT1SN) (crosses) and control cells (MC3T3-E1-LXSN (LXSN)) (squares) cells were seeded at 20,000 cells/cm2 in 4-well plates and counted at the indicated days. The results are shown as mean cell number per cm2 of 4 wells ± SD. *indicates statistically significantly different from MC3T3-E1-LXSN cells at the same day, p <0.05. B and C) Cell cycle analysis. MC3T3-E1-LPiT1SN (LPiT1SN), MC3T3-E1-LXSN (LXSN), and MC3T3-E1-LPiT2SN (LPiT2SN) were seeded at 20,000 cells/cm2 in T25-flasks, stained with propidium iodide 24 hours later, and analyzed by flow cytometry. B) Representative flow cytometry histograms of MC3T3-E1-LXSN (LXSN) (top), MC3T3-E1-LPiT1SN (LPiT1SN) (middle), and MC3T3-E1-LPiT2SN (LPiT2SN) (bottom) cells; the thick black lines represent the Watson-Pragmatic model. C) The percentages of cells in the respective phases of the cell cycle obtained using the Watson Pragmatic model. Each column represents the mean of triplicate set ups ± SD. *indicates statistically significantly different from MC3T3-E1-LXSN (control) cells in the same phases of the cell cycle, p < 0.05.