Figure 7
From: Regulation of cell proliferation and cell density by the inorganic phosphate transporter PiT1
Effect of over-expression of hPiT1 in NIH3T3 cells on the endogenous mPiT1 and mPiT2 mRNA levels and P i uptake. A and B) qRT-PCR analyses of endogenously expressed mPiT1 and mPiT2. NIH3T3-LXSN (LXSN) and NIH3T3-LPiT1SN (LPiT1SN) cells were seeded at 20,000 cells/cm2 in 4-well plates and the endogenous mPiT1 (A) and mPiT2 (B) mRNA levels were analyzed by qRT-PCR at indicated days in culture. Each column represents cell lysates from three wells and triplicate qRT-PCR analyses of each cell lysate. The mPiT1 and mPiT2 mRNA levels are standardized to B2M mRNA levels. Data are means ± SD. * indicates statistically significantly different from NIH3T3-LXSN (control) cells at the same day, p < 0.05. E) Pi-transport assay. NIH3T3-LXSN (LXSN) and NIH3T3-LPiT1SN (LPiT1SN) cells were seeded at 20,000 cells/cm2 in 4-well plates. The next day, 32Pi import in medium containing a total [Pi] of 100 μM was analyzed over 5 minutes. The results are shown as mean 32Pi import per mg protein per hour of 4 wells ± SD. * indicates statistically significantly different from NIH3T3-LXSN (control) cells, p < 0.05.