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Table 1 Transduction study analyzing the presence of functional hPiT1, hPiT2, and mPiT2 on the cell surface of MC3T3-E1-LXSN, -LPiT1SN, -LPiT2SN, NIH3T3-LXSN, and - LPiT1SN cells

From: Regulation of cell proliferation and cell density by the inorganic phosphate transporter PiT1

Cells a)

Pseudotype of vectors encoding LacZ

 

A-MLV b)

GALV

MC3T3-E1-LXSN

4.7 × 104 c)

0 d)

MC3T3-E1-LPiT1SN

4.5 × 104

4.3 × 103

MC3T3-E1-LPiT2SN

6.5 × 104

0

NIH3T3-LXSN

6.4 × 105

0

NIH3T3-LPiT1SN

5.4 × 105

3.6 × 104

  1. a) MC3T3-E1 cells transduced with empty vector (LXSN), LPiT1SN, or LPiT2SN retroviral vectors were challenged with retroviral vectors pseudotyped with A-MLV or GALV surface proteins and carrying a β-galactosidase encoding transfer vector.
  2. b) The titers on D17 cells of the vector stocks used were 1.7 × 105 CFU/mL (A-MLV pseudotype) and 1.9 × 105 CFU/mL (GALV pseudotype).
  3. c) Numbers indicate blue CFU per mL vector containing supernatant.
  4. d) No blue cells were detected in the wells.
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Cell Division

ISSN: 1747-1028

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