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Table 2 Colony formation by NIH3T3-LXSN and NIH3T3-LPiT1SN cells in soft agar

From: Regulation of cell proliferation and cell density by the inorganic phosphate transporter PiT1

Cells NCS a) FBS b)
NIH3T3-LXSN 0.5 ± 0.5c) 69.0 ± 8.3
NIH3T3-LPiT1SN 2.5 ± 1.8 97.5 ± 9.8d)
  1. a) The cells were cultured in normal growth medium prior to as well as during cultivation in soft agar (DMEM supplemented with 10% NCS and 1% PS).
  2. b) The cells were cultured in transformation medium 2 days prior to as well as during cultivation in soft agar (DMEM supplemented with 10% FBS and 1% PS).
  3. c) Numbers indicate mean numbers of colonies per well of 4 wells ± SD.
  4. d) Statistically significantly different from colonies derived from NIH3T3-LXSN (control) cells grown in FBS, p < 0.05.