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Table 2 Colony formation by NIH3T3-LXSN and NIH3T3-LPiT1SN cells in soft agar
Regulation of cell proliferation and cell density by the inorganic phosphate transporter PiT1
0.5 ± 0.5
69.0 ± 8.3
2.5 ± 1.8
97.5 ± 9.8
a) The cells were cultured in normal growth medium prior to as well as during cultivation in soft agar (DMEM supplemented with 10% NCS and 1% PS).
b) The cells were cultured in transformation medium 2 days prior to as well as during cultivation in soft agar (DMEM supplemented with 10% FBS and 1% PS).
c) Numbers indicate mean numbers of colonies per well of 4 wells ± SD.
d) Statistically significantly different from colonies derived from NIH3T3-LXSN (control) cells grown in FBS, p < 0.05.
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