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Figure 8 | Cell Division

Figure 8

From: lemmingA encodes the Apc11 subunit of the APC/C in Drosophila melanogaster that forms a ternary complex with the E2-C type ubiquitin conjugating enzyme, Vihar and Morula/Apc2

Figure 8

LmgA interacts with APC2/Mr and they together interact with the E2 enzyme Vihar. In yeast two-hybrid experiments, S. cerevisiae L40 strain was co-transformed with apc2-pGAD424 and lmgA-pBTM116 (A1), apc2-pBTM116 and lmgA-pGAD424 (A2), apc2-pBTM116 and lmgAB-pACT2 (A3), as well as with apc2-pBTM116 and lmgB-pGAD424 (A4) plasmids. Blue colonies indicate the interaction. In yeast three hybrid experiments, Tat7 cells were co-transformed with lmgA-pBTM116, apc2-pRS416 and pGAD424 (B1), lmgA-pBTM116, apc2-pRS416 and vihar-pGAD424 (B2), lmgA-pBTM116, apc2-pRS416 and vihar-C-term-pGAD424 (B3), and lmgA-pBTM116, apc2-pRS416 and lmgB-pGAD42 (B4) and PXG-assays were performed. Strong interactions are indicated by dark blue colorations in columns 2 and 3. The three rows represent parallel experiments. Beta-Glo® assays were performed with the same transformants grown in liquid minimal medium, in the presence or absence of 1 mM methionine (C). Increased luminescence in lmgA-Apc2-vihar and lmgA-Apc2-viharC-term indicates interactions.

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