Figure 3
Expression of cell cycle regulators was upregulated by ISO but abolished by β-AR antagonists. A, Cell cycle analysis was performed to determine the different phases of the cell cycle that occurred when HemECs were treated with or without ISO, MET or ICI. The data shown were collected from 10,000 events. B, Histogram distribution of cells in the G0/G1, S and G2/M phases as determined by flow cytometry. C, Western blot analysis of cyclin and CDK protein expression. The expression of cyclin D1, CDK-4 and CDK-6 was upregulated by ISO but abolished by MET or ICI. GAPDH served as the loading control. D, Western blot analysis of Rb phosphorylation. ISO caused the phosphorylation of Rb, and this effect was inhibited by MET or ICI. Total Rb (tRb) was used for normalization. Data are representative of three independent experiments. * P<0.05 when compared with the ISO-untreated control, † P<0.05 when compared with the ISO-treated control.