Figure 6
Effect of β-AR stimulation on ERK activation in HemECs. A, HemECs were treated with ISO for various times (0-60 min), and ERK phosphorylation was detected by Western blotting. ERK phosphorylation peaked 30 min after ISO treatment. B, HemECs were pre-treated with MET (10 μM), ICI (10 μM) or PTK787 (10 μM) for 1 h and incubated with ISO (1 μM) for 30 min. The phosphorylation status of ERK was then detected. Total ERK was used as an internal control. The experiments were repeated three times, and similar results were obtained. * P<0.05 when compared with the ISO-untreated control, † P<0.05 when compared with the ISO-treated control.